China Buffered Peptone Water Manufacturer & Suppliers

Premium Grade Dehydrated Culture Media & Fermentation Ingredients Formulated to International Regulatory Standards

Established 2008

About Us

Beijing Hongrun Baoshun Technology Co., Ltd. is located in the high-tech Science and Technology Innovation Area of the China (Beijing) Pilot Free Trade Zone.

We have evolved into a modern high-tech enterprise integrating R&D, production, and sales. We are proud recipients of dual certifications as a National "High-tech Enterprise" and a "Zhongguancun High-tech Enterprise."

Our strategically optimized operations expanded in 2018 to the Qili Industrial Park in Langzhong, Sichuan. With Phase I and Phase II factories, our combined floor area exceeds 40,000 square meters, backed by a modern 20,000 square meter storage facility. Our advanced facility isolates animal and plant-derived production lines, eliminating cross-contamination risk.

2008
Established Year
40,000+
Factory Area (㎡)
20,000+
Storage Volume (㎡)
Hongrun Bio Facility

ISO 9001:2015

Strict Quality System Certification

MUI HALAL

Global Dietary Compliance Standard

Production Capabilities

Advanced Production Line & Logistics Centers

A visual insight into our state-of-the-art facilities, featuring separate processing systems for raw agricultural input processing and animal-derived protein extraction.

Production Facility 1
Production Facility 2
Production Facility 3
Global Vision
Collaborative Strength
Forge Ahead Future

The Biochemistry & Technical Standards of Buffered Peptone Water (BPW)

In food safety, clinical diagnostics, and environmental testing, the recovery of sublethally injured pathogens is crucial. Buffered Peptone Water (BPW) is a non-selective pre-enrichment medium designed to optimize cell recovery. Standard food processing steps—such as heat treatments, desiccation, exposure to preservative acids, and chemical sanitization—often stress target organisms like Salmonella species and Listeria monocytogenes without killing them. These damaged cells cannot grow directly on selective isolation media due to the toxic selective agents used. A non-selective recovery step is required to resuscitate them.

Optimal Buffering & Nutritional Formula

The standard formulation of BPW relies on a precise stoichiometric balance of peptones, osmotic regulators, and phosphate buffers. High-quality enzymatic digests of casein (peptone) provide essential nitrogen, free amino acids, carbon, vitamins, and minerals required for bacterial metabolism. Sodium chloride maintains osmotic equilibrium, preventing cellular lysis. The primary buffer system uses disodium hydrogen phosphate and potassium dihydrogen phosphate. This system maintains the pH of the medium at 7.2 ± 0.2 at 25°C throughout pre-enrichment. This pH stability is critical when recovering acid-producing organisms or analyzing naturally acidic foodstuffs, which can cause pH drops that kill stressed pathogens.

Regulatory Standards: ISO 6579 & FDA-BAM

Global trade regulations mandate standardized pathogen testing. ISO 6579 (Microbiology of the food chain - Horizontal method for the detection, enumeration, and serotyping of Salmonella) specifies BPW as the primary pre-enrichment medium. Similarly, the FDA Bacteriological Analytical Manual (BAM) and the European Pharmacopoeia require BPW for testing food matrices, animal feeds, and environmental samples. Batch-to-batch consistency in buffer capacity, productivity ratios (PR ≥ 0.5), and low background contamination are critical quality control parameters. These parameters are rigorously validated under ISO 11133 quality standards.

Market Evolution & Global Procurement Requirements

The global demand for dehydrated culture media is shifting due to regulatory changes, animal welfare concerns, and industrial efficiency needs. B2B buyers must navigate these changes to maintain clean, compliant, and cost-effective supply chains.

The Shift to Plant-Derived (Vegan) Alternatives

One major trend in microbial media manufacturing is the transition from animal-derived peptones to plant-derived alternatives. This shift is driven by the need to eliminate risks associated with Bovine Spongiform Encephalopathy (BSE) and Transmissible Spongiform Encephalopathies (TSE). As a result, plant proteins like soy and wheat are widely used to replace animal peptones. Our Sichuan facility has separate production lines for plant and animal peptones, ensuring zero cross-contamination and providing certified BSE/TSE-free plant-derived raw materials for global pharmaceutical, cosmetic, and vaccine manufacturers.

Supply Chain Resilience & Customization

Microbiology laboratories and industrial fermentation facilities require reliable supply partners. B2B procurement professionals must balance cost, consistent raw material supply, and batch scalability. As a vertically integrated manufacturer, Beijing Hongrun Baoshun Technology Co., Ltd. addresses these challenges by managing the entire manufacturing process—from raw material selection to enzymatic hydrolysis, spray drying, and packaging. This end-to-end control ensures consistent bulk supply, competitive pricing, and custom formulation options to meet specific customer requirements.

ISO 9001:2015 Approved
MUI Halal Compliant
Custom Hydrolysis Services
Separate Animal/Plant Lines
Synergetic B2B Network

Our Multi-Divisional Subsidiary Structure

Beijing Hongrun Baoshun Technology Co., Ltd. operates with five specialized subsidiaries. This structure enables synergetic development and ensures end-to-end quality control across our supply chain.

Beijing Yaoyou Technology

Coordinates our biotechnology research and development efforts, focusing on optimizing microbial yield and culture medium formulations.

Sichuan Baoshun Biotech

Manages our large-scale Sichuan production facility, focusing on animal-derived proteins and raw peptone extraction processes.

Sichuan Romgee Biotech

Focuses on downstream application testing, validating final product quality using modern microbiology laboratory assays.

Sichuan Yingwen Tech

Manages our domestic and global regulatory compliance, handling certifications, import-export paperwork, and customs coordination.

Sichuan Runxiaobao Tech

Coordinates domestic sales networks, customer service, technical support, and logistics for the Chinese market.

Diverse Formulations

End-Use Application Profiles

Our raw materials and dehydrated culture media support critical microbiology applications, including clinical diagnostics, food safety testing, and industrial biotechnology.

Culture Media Formulations

Widely applied in formulating dehydrated media to support microbial growth and identification.

Biomedical Applications

Provides critical nutrients for vaccines, recombinant proteins, and therapeutic cell cultures.

Biochemical Engineering

Used as high-nitrogen fermentation nutrients to optimize target molecule yields in bioreactors.

Probiotics & Microecologicals

Supports the growth of beneficial microorganisms like lactic acid bacteria and bifidobacteria.

Biological Agriculture

Provides nutritional inputs for organic fertilizers and biopesticides, enhancing crop health.

Enzyme Preparations

Optimizes nutrient feeds to stimulate enzyme expression in industrial fermentations.

Tailored Product Development

Product Customization Services

We offer customizable formulations to meet the specific requirements of our clients. Let us design a solution tailored to your operational needs.

Our Sichuan facility features separate plant and animal processing lines, enabling us to customize products to your exact requirements. We can adjust the molecular weight distribution of peptones, modify amino acid profiles, and customize packaging sizing to integrate into your production processes.

We provide a comprehensive range of high-quality, stably supplied fermentation raw materials backed by independent production and free sampling.

Free Sampling Program

We offer free samples of up to 500g per product (excluding shipping fees). This program allows laboratories and manufacturers to validate raw materials in their own systems before committing to bulk purchases.

Hongrun Customization
Operational Excellence

Why Partner With Us?

Our commitment to quality control, technical support, and supply chain management ensures reliable, long-term partnerships for global laboratories and manufacturers.

Professional Technical Support

01. Professional Technical Support

We provide full-cycle technical support, including pre-sales consultation, on-site debugging, after-sales maintenance, and timely problem-solving to support the stable operation of your system.

Customized Service Solutions

02. Customized Service Solutions

We offer customization tailored to your business needs, covering product design, functional development, packaging, and application solutions to meet diverse requirements.

Stable & Reliable Product Quality

03. Stable & Reliable Quality

Strict quality control systems and standardized production processes ensure consistent product performance, high purity, and reliability for long-term customer trust.

Efficient Logistics

04. Efficient Logistics & Timely Delivery

Integrated supply chain management and multiple warehousing distribution centers support fast order processing and on-time delivery, reducing lead times.

Competitive & Flexible Pricing

05. Competitive & Flexible Pricing

We offer cost-effective pricing strategies and flexible cooperation models, supporting bulk discounts and long-term partnerships.

Multilingual Professional Service

06. Multilingual Professional Service Team

Our professional service team offers multilingual support to facilitate clear, barrier-free communication and business cooperation for global clients.

Comprehensive After-Sales

07. Comprehensive After-Sales Service Guarantee

We offer a structured after-sales service system, including return and exchange policies, technical training, and regular follow-up visits to support your ongoing operations.

Information Hub

Frequently Asked Questions

Common technical and commercial questions about Buffered Peptone Water sourcing, standards compliance, and custom biological raw materials.

What is the shelf life and optimal storage condition of dry Buffered Peptone Water?
Our dehydrated Buffered Peptone Water (BPW) typically offers a shelf life of 36 months from the date of manufacture. It must be stored in tightly sealed containers in a cool, dry place at temperatures between 10°C and 30°C, away from direct sunlight. Due to the hygroscopic nature of peptones, humidity control is essential to prevent caking and clumping.
How do you guarantee the separation of animal and plant-derived production lines?
Our Langzhong, Sichuan production facility is designed with dedicated, physically isolated processing rooms, air filtration systems, and manufacturing lines for animal-derived and plant-derived (vegan/BSE-free) proteins. This design prevents cross-contamination, enabling us to provide certified vegetable-derived peptones for sensitive industrial applications.
Does your Buffered Peptone Water comply with the ISO 6579 standard?
Yes, our Buffered Peptone Water is formulated and batch-tested to comply with the performance requirements of ISO 6579 and ISO 11133. Each batch is verified for microbiological productivity, selectivity, and physical-chemical parameters (such as pH stability at 7.2 ± 0.2). Detailed Certificates of Analysis (CoA) are provided with every shipment.
What certifications support your manufacturing operations?
Our company is ISO 9001:2015 certified, establishing structured quality control from raw material inspection to final product dispatch. In addition, we hold MUI Halal certification, supporting compliance with dietary regulations in global markets.

Technical Specification & Preparation Protocols

To support laboratory operations, we provide preparation parameters and performance standards for our Buffered Peptone Water. Adherence to these guidelines helps maintain analytical reproducibility across food testing and clinical laboratories.

Ingredient Component Standard Concentration (g/L) Primary Biological Function
Enzymatic Digest of Casein (Peptone) 10.0 Provides nitrogen, vitamins, carbon, and essential amino acids for growth.
Sodium Chloride (NaCl) 5.0 Maintains osmotic pressure, preventing cellular damage during pre-enrichment.
Disodium Hydrogen Phosphate (Na₂HPO₄) 3.5 Maintains buffering capacity, stabilizing the pH of the medium.
Potassium Dihydrogen Phosphate (KH₂PO₄) 1.5 Works with disodium hydrogen phosphate to maintain a stable neutral pH.

Dehydrated Preparation Protocol:

  • Suspend 20.0 grams of dehydrated Buffered Peptone Water powder in 1 liter of purified or distilled water.
  • Mix thoroughly and heat to dissolve the powder completely.
  • Dispense the solution into final containers (such as flasks or dilution bottles).
  • Autoclave the containers at 121°C for 15 minutes to sterilize.
  • Allow the media to cool to room temperature before inoculation. Verify that the final pH is 7.2 ± 0.2 at 25°C.

Quality Assurance and Performance Control:

Our quality control processes validate each batch against test strains, including Salmonella typhimurium (ATCC 14028) and Salmonella enteritidis (ATCC 13076). resuscitating sublethally injured cells within 18–24 hours at 37°C. The formulation is also tested to ensure low bioburden levels, minimizing background growth that can interfere with diagnostic assays.